Vo Thanh Sang
Nguyen Tat Thanh University, Vietnam
Title: Spirulina maxima Peptides as Potential Biomaterials for Alternative Medicine
Biography
Biography: Vo Thanh Sang
Abstract
Recently, a great deal of interest has been developed by consumers towards bioactive peptides as potential biomaterials. In this study, two bioactive peptides derived from gastric enzymatic hydrolysate of Spirulina maxima including P1 (LDAVNR; 686 Da) and P2 (MMLDF; 655 Da) have been determined for its anti-oxidant, anti-allergy, and anti-inflammation in vitro. For antioxidant activity, peptides P1 and P2 have been evidenced to be effective in scavenging DPPH and ABTS+ radicals. These peptides exhibited protective effect against DNA damage induced by oxidant H2O2. Moreover, intracellular reactive oxygen species (ROS) production was reduced in RBL-2H3 mast cells and EA.hy926 endothelial cells as the cells treated with peptides P1 or P2. For anti-allergic activity, P1 and P2 were found to be effective against FcεRI-mediated RBL-2H3 mast cell activation via decreasing histamine and β-hexosaminidase release and IL-4 and IL-13 productions. The inhibitory activity of P1 was found to be due to decrease mast cell activation via blockade of FcεRI-IgE interaction, while P2 was observed to suppress PLCγ activation, thus attenuating intracellular calcium elevation and mas cell degranulation. For anti-inflammatory activity, P1 and P2 exhibited inhibitory activities on the production and expression of IL-6, IL-8, and monocyte chemoattractant protein-1 in EA.hy926 endothelial cells induced by histamine. Furthermore, P1 and P2 suppressed the production of adhesion molecules via down-regulating Egr-1 expression. Taken together, both peptides P1 and P2 from Spirulina maxima have been identified as great potential biomaterials for alternative medicine application against allergic and inflammatory responses.